The objective of this study was to investigate the biodegradation of ethylene in an biofilter inoculated with ethylene-oxidizing microorganisms. The biofilter performance was monitored in terms of ethylene removal efficiency and carbon dioxide production. The biofilter was capable of achieving the ethylene removal efficiency as much as 100% at a residence time of 14 min and an inlet concentration of 290 ppm. Under the same conditions, carbon dioxide with a concentration of up to 546 ppm was produced. It was found that carbon dioxide was produced at a rate of 87 mg/day, which corresponded to a volume of 0.05 L/day. Observable features of the ethylene-oxidizing microorganisms, meaning microbial activity occurrence in the biofilter, were investigated with the microscopy analysis.