This study has examined effects of protease on the removal of hemoglobin and triolein mixed soil with changes of soil content and soil composition from cotton and PET filament fabrics. The results obtained were as follows: The results obtained were as follows: 1) The detergency of PET fabrics was higher than that of cotton fabrics. The removal of hemoglobin was much higher than that of triolein from cotton fabrics, while the removal of hemoglobin was similar to that of triolein from PET fabrics. 2) The removal efficiency of hemoglobin and triolein was improved by protease from cotton and PET fabrics. Especially the removal efficiency of those was remarkably improved from cotton fabrics which the removal of soil was lower than PET fabrics. And the removal of hemoglobin and triolein by adding protease was increased with increase of hemoglobin content of mixed soil from cotton fabrics. 3) With the increase of hemoglobin content of mixed soil, the removal of hemoglobin was drastically increased but the removal of triolein was slightly decreased from cotton and PET fabrics. With the increase of triolein content of mixed soil, the removal of hemoglobin and triolein was decreased from cotton fabrics, but those were generally increased from PET fabrics.