The phenomena of energy separation in vortex tubes was investigated experimentally to see the effects of the conductivity of a tube and convective heat transfer on the outer surface of a tube. The experiment was carried out with different conductivity (pyrex, stainless steel and copper) of a tube and three kinds of convective heat transfer modes (adiabatic condition, natural convection (air) and forced convection (water) on the outer surface of a tube. the results were obtained that hot exit fluid temperature was highly affected by a change of conductivity of a tube when the outer surface was cooled by the forced convection of water. However, the cold exit temperature was little affected by heat transfer modes on the outer surface in vortex tubes.