Constant ΔK fatigue crack growth tests were performed by applying an intermediate multiple overload for S45C steel. The purpose of the present study is to investigate effects of specimen thickness at various baseline stress intensity factor range levels (ΔK(sub)b), overload application position (a/W) and overload application frequency (OL(sub)HZ) on fatigue crack growth retardation behavior. The principal results are summarized as follows. The amount of retardation for a given ΔK(sub)b level is increased with increasing the baseline stress intensity factor range level for all specimen thickness. The normalized minimum crack growth rate is increased with increasing the specimen thickness, except for ΔK=45MPa√m. The retardation cycle is decreased with increasing a/W and increased with OL(sub)HZ.