Psychological and physiological effects from simulator sickness could be an important bias factor for sensibility evaluation. The present experiment investigated the effects of simulator sickness on sensibility evaluation in the controlled condition of driving a car for 60 minutes on a constant speed (60km/h) in graphic simulator. The simulator sickness was measured and analysed for every five minutes using their subjective evaluation and physiological signals. Results of the subjective evaluation showed that there was significant difference between rest and driving condition at 10 minutes from the start of driving, and the level of difference was increased linearly with time. The analysis on central and autonomic nervous systems showed the significant difference between rest and driving conditions after 5 minutes from the start of the driving on the parameters $alpha$/total and $eta$/total, and increased level of sympathetic nervous system. But there was no significant difference between different time conditions. The results indicates that physiological changes from simulator sickness can be a bias factor in objective evaluation of human sensibility which also, uses physiological signals. That is, the changes on the parameter $alpha$/total and $eta$/total, and on activation level of sympathetic nervous system from simulator sickness can be a bias factor for evaluation of the level of pleasantness and tension. Therefore the effort on improving the analysis by minimizing or eliminating the bias factors should be done for better and accurate sensibility evaluation in simulator environments.