The laminar convection heat transfer in a ventilated space with various horizontal partitions was studied numerically and experimentally. The experimental results by holographic interferometer showed good agreements with the numerical results. For the numerical study, the governing equations were solved by using a finite volume method for $46.6leqReleq1332,;1460leqGrleql1585$, Pr=0.71 and the variations of partition lengths. The isotherms and velocity vectors have been represented for various parameters. As the length of partition increases, the convection heat transfer decreases. Based on the numerical data, a correlation was obtained for the dimensionless mean Nusselt number in terms of $Gr/Re^2$. In the region of $Gr/Re^2$<1, the mean Nusselt number was very small, but in the region of $Gr/Re^2geq1$, the mean Nusselt number was constant.