- 페놀분해세균인 Pseudomonas sp. EL-04J에 의한 Trichloroethylene의 공동대사
- ㆍ 저자명
- 김호성,박근태,손홍주,박성훈,이상중
- ㆍ 간행물명
- 한국환경과학회지
- ㆍ 권/호정보
- 2001년|10권 5호|pp.359-364 (6 pages)
- ㆍ 발행정보
- 한국환경과학회
- ㆍ 파일정보
- 정기간행물| PDF텍스트
- ㆍ 주제분야
- 기타
Pseudomanas sp. EL-04J was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). After precultivation in the mineral salts medium containing phenol as a sole carbon source, Pseudomonas EL-04J degraded 90% of TCE $25 mu extrm{M}$ within 20 hours. Thus, phenol-induced Pseudomonas sp. EL-04J cells can bdegrade TCE. Followsing a transient lag period, Pseudomonas sp. EL-04J cells degraded TCE at concentrations of at least $250 mu extrm{M}$ with no apparent retardation in rate, but the transformance capacity of such cells was limited and depended on the cell concentration. The degradation rate of TCE followed the Michaelis-Menten kinetic model. The maximum degradation ratio ($V_{max}$) and saturation constant ($K_{m}$) were $7nmo {ell}/min{cdot}mg$ cell protein and $11 mu extrm{M}$, respectively. Cometabolism of TCE by phenol fed experiment was evaluated in $50m {ell}$ serum vial that contained $10m {ell}$ of meneral sals medium supplemented with $10 mu extrm{M}$ TCE degradation was inhibited in the initial period of 1 mM phenol addition, but after that time Pseudomonas sp. EL-04J cells degraded TCE and showed cell growth.