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페놀분해세균인 Pseudomonas sp. EL-04J에 의한 Trichloroethylene의 공동대사
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  • 페놀분해세균인 Pseudomonas sp. EL-04J에 의한 Trichloroethylene의 공동대사
저자명
김호성,박근태,손홍주,박성훈,이상중
간행물명
한국환경과학회지
권/호정보
2001년|10권 5호|pp.359-364 (6 pages)
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한국환경과학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Pseudomanas sp. EL-04J was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). After precultivation in the mineral salts medium containing phenol as a sole carbon source, Pseudomonas EL-04J degraded 90% of TCE $25 mu extrm{M}$ within 20 hours. Thus, phenol-induced Pseudomonas sp. EL-04J cells can bdegrade TCE. Followsing a transient lag period, Pseudomonas sp. EL-04J cells degraded TCE at concentrations of at least $250 mu extrm{M}$ with no apparent retardation in rate, but the transformance capacity of such cells was limited and depended on the cell concentration. The degradation rate of TCE followed the Michaelis-Menten kinetic model. The maximum degradation ratio ($V_{max}$) and saturation constant ($K_{m}$) were $7nmo {ell}/min{cdot}mg$ cell protein and $11 mu extrm{M}$, respectively. Cometabolism of TCE by phenol fed experiment was evaluated in $50m {ell}$ serum vial that contained $10m {ell}$ of meneral sals medium supplemented with $10 mu extrm{M}$ TCE degradation was inhibited in the initial period of 1 mM phenol addition, but after that time Pseudomonas sp. EL-04J cells degraded TCE and showed cell growth.