The experimental investigation on energy separation in a vortex tube has been carried out to sow the effect of inlet and outlet pressures with various working fluids(air,$O_2,;and; CO_2$). Those outlet pressure means cold outlet and hot outlet pressure which were set equally. The results showed that the total enthalpy variation became a maximum when the mass flow rate at the cold outlet was a half of the total mass flow rate in the vortex tube (y=0.5). The total enthalpy variation was quite affected by the pressure difference between the inlet and outlet of vortex tube when the ratio of the inlet pressure to the cold outlet pressure remained constant. Although specific enthalpy differences between the inlet and the outlet (both cold and hot outlet) did not noticeably vary with the pressure difference, the specific enthalpy difference between the inlet and cold outlet was dominantly affected by physical properties of working gases.