The present experiment investigated the possibility of evaluating of the human sensibility contingent on the speed and modes of driving using the responses from the autonomic nervous system, subjective assessments, Simulator Sickness (55) in dynamic simulator. The three conditions of the speed of driving were 40 $pm$ 10 km/h, 100$pm$10 km/h, 160 $pm$ 10 km/h, and the participant was instructed to drive the car for three minutes on the elliptical track. It is programed in such a way that the modes of driving can be changed smoothly using road DB in Dynamic Simulator, and for signifying the change of the condition the road signs were used. The instruction was given to the participant to drive the car on the fixed speed of 20 km/h for 30 seconds, then to drive the car on sudden-start mode of driving from the 20 km/h to 160 km/h within 10 seconds. For the sudden-stop mode of driving, it was instructed that stop the car from the speed of 160km/h to 20km/h within 10 seconds when the subject see the road sign, then drive the car at the fixed speed of 20 km/h for 30 seconds. The results of the subjcetive assessment showed that the level of pleasantness and the tension was increased, and physiological response showed that the level of activity of the autonomic responses were also increased as the speed of the car increased. Also, for results on the driving modes showed that the level of pleasantness was highest for the sudden-stop, next highest was sudden-start, and the lowest was 20 km/h fixed speed condition for the subjective assessment, and tile order of the level of activation of the autonomic nervous system showed the same results as above. From the results of the present study it was concluded that the presentation and evaluation of the stimulus for the human sensitivity is possible in dynamic simulator.