In this paper, we present a new cost function for subband block adaptive algorithm and block conjugate gradient algorithm for noise cancellation of acoustic signal. For the cost function, we process the subband signals with data blocks for each subbands and recombine it a whole data block. After these process, the cost function has a quadratic form in adaptive filter coefficients, it guarantees the convergence of the suggested block conjugate gradient algorithm. And the block conjugate gradient algorithm which minimizes the suggested cost function has better performance than the case of full-band block conjugate gradient algorithm, the computer simulation results of noise cancellation show the efficiency of the suggested algorithm.