Distribution of fuel-air mixture has a strong influence on performance and emissions of a compressed natural gas (CNG) engine. In this paper, parametric study is performed by KIVA-3V to investigate fuel-air mixture with respect to injection timing, cycle equivalence ratio and engine speed. With open-valve injection intensive mixing during intake and compression stroke results in relatively homogeneous mixture in the cylinder. Sequential induction of fuel-air mixture and fresh air results in stratification in the cylinder among the test cases at closed-valve injection. There is close similarity in the calculated distributions of the mixture in the cylinder with different cycle equivalence ratios and engine speeds. The results are compared against pressure traces and flame images obtained in a single cylinder engine converted from a 11L six-cylinder heavy duty diesel engine.