Aims of study : The purpose of this study is to measure the chronic and daily voice burden of the professionals in their actual working places. These will be a valuable guideline for preventing and controlling the voice production of professionals. Material and method : Our study was selected to the 10 female telephone operators in the Asan Medical Center, ages ranging from 22 to 38 years old. The symptoms and acoustic analysis of both telephone operators and the controls were evaluated before and after their working. The symptoms were evaluated with questionaires, and the acoustic analysis was measured by using CSL (computerized speech laboratory) system. Results : The symptoms of the professional voice abusers are same as those symptoms in laryngeal fatigue. The acoustic analysis before their working were significantly increased in jitter and shimmer, in comparison with the data of the control. This shows that the experimental group is exposed to the chronic burden of voice production. The jitter, shimmer, and NHR after their working are significantly increased in comparison with the data of the acoustic analysis before their working. This also shows that the experimental group is exposed to the daily burden of voice production. Conclusion : The acoustic analysis of the professional voice overusers has objectively measured that there are chronic and daily overloading to the voice of operators, and these will be a valuable data for preventing and controlling the professionals that abuse their voice.