In this paper, the method is suggested to prevent data collision or damage on RFID(Radio Frequency Identification) system, in case a reader reading multi-tag simultaneously, using binary-search algorithm and Time-domain anti-collision procedure at reader and tag, respectively. The RFID system is designed that Reader enable to communicate with Tag on 13.56MHz bandwidth which is ISM(Industrial Science Medical) bandwidth, antennas of Tag part are designed using MCRF335 Chip. When RF communication is achieved between reader and tag, in case that data is transmitted to reader pass through multiple tags simultaneously, a study on the anti-collision method for the situation that the data collision occurs is performed.