The purpose of this study was to investigate the of regular worksite health exercise program participation on related fitness. Subject for study were 34 middle-aged women in Kwangju-city. To achieve this, body composition, cardiopulmonary function, physical fitness of every subjects were measured before and post regular health exercise program participation during 12 week. The results of this study were as follows: 1. Regular health exercise program participation result from improving the body composition, but there is not significant different statistically(p〈.05). 2. Significant differences were observed in the cardiopulmonary function(vital capacity, VO$_2$ max) after regular exercise program during 12 week(p〈.05). 3. Significant differences were observed in the flexibility(sitting trunk flexion), abdominal endurance(sit-ups), power (standing high jump), agility(trunk reaction time) after regular exercise program during 12 week(p〈.05).