The purpose of this study is to analyze the problems Korean students, having acoustic structure of Korean vowels, have when they pronounce English vowels by measuring formant frequencies. The experimental results show that the pronunciation of English vowels by Korean students is partially influenced by their Korean vowels. There is little distinction between /i/ and /I/, /U/ and /u/ due to the absence of short and long vowels in Korean pronunciation. Also, as observed in typical Korean vowel pronunciation, there is little difference between the F1 values of /$varepsilon$/ and /${ae}$/ by Korean speakers, resulting in inaccurate English pronunciation. In addition, compared to English native speakers, Korean speakers show the biggest difference in F1 value of /c/. The fact that they make pronunciation of /c/ covering /e/, /$Lambda$/ and /c/ positions probably accounts for such phenomenon. The results of this experiment show the interference of Korean that occurred in some English vowels by native Korean speakers.