The computerized cardiac analysis system, which acquires and analyzes the electrical activation signal propagating along the surface of the heart, is indispensible equipment for the open heart surgery and electrical cardiac study. In this paper, the design requirement and the electrical circuit analysis are performed to construct the multi-channel cardiac activation pre-amplifier necessary for a signal conditioning circuit. The general 64 channel configuration is expanded into 128 channels to enhance the spatial resolution on the mapped surface of the heart. The 128 channels pre-amplifier consists of input circuit, differential amplifier, right leg driven circuit and isolation part. It has distinct features; high voltage protection, leakage current limitation, isolation and the maximization of common mode rejection ratio with respect to the half-cell potential difference due to different electrode materials. The final pre-amplifier circuit is assembled with 8 boards, each of which composing of 16 channels.