This paper deals with speed control of DC servo motor using a PID controller with a gain tuning based on a Back-Propagation(BP) Learning Algorithm. Conventionally a PID controller has been used in the industrial control. But a PID controller should produce suitable parameters for each system. Also, variables of the PID controller should be changed according to environments, disturbances and loads. In this paper described by a experiment that contained a method using a PID controller with a gain tuning based on a Back-Propagation(BP) Learning Algorithm, we developed speed characteristics of a DC servo motor on variable loads. The parameters of the controller are determined by neural network performed on on-line system after training the neural network on off-line system.