Study the variation of low frequency electrical stimulation on human metabolism which serum to test the increasement of creatine- kinase, myoglobin, lactate .and pyruvate. The results were as followed: 1) Creatine kinase increasement showed significant difference between 200 Hz stimulation group and control group, and also between 300Hz stimulation group and control group, but no significant increasement difference showed in 50 Hz stimulation group compared to control group. 2) Lactate increasement showed significant difference in 200 Hz stimulation group and 300 Hz stimulation group compared to control group, but no significant difference showed in 50 Hz stimulation group. 3) Pyruvate increasement showed significant difference in 50 Hz stimulation group and 200 Hz stimulation group compared to control group, but no significant increasement difference showed between 50 Hz stimulation group and control group. (P < 0.05) 4) Myoglobin increasement showed significant difference in 50Hz stimulation group and 200 Hz stimulation group compared to control group, and also in 200 Hz stimulation group and 300 Hz stimulation group compared to 50Hz stimulation group. ( P < 0.05)