In this study, the effect of specimen thickness and stress ratio on fatigue crack growth in S45C steel was investigated. Acoustic emission was monitored during the fatigue crack growth test. Both crack closure and AE technique were used in assessing fatigue crack growth behavior. Constant amplitude loading tests were performed on CT type specimen with three different thicknesses and stress ratios. Crack closure was investigated to explain the influence of specimen thickness and stress ratio on the fatigue crack growth in the second growth region. The crack closure effect was decreased with specimen thickness and stress ratio.