To evaluate the effect of disinfectants for Alginate impression, several commercially available disinfectants were tested to Alginate model contaminated by Methicillin resistant Staphylococcus aureus. Alginate specimens were artificially contaminated by Staphylococcus aureus and soaking in four commercially available disinfectants for 1, 5, 10 minutes and colonies were counted for each case. Significant reduction of bacterial colony counts was noted after soaking in 0.5% and 0.05% sodium hypochlorite and after soaking in 2% glutaraldehyde, but not after soaking in Hypochlorus add. Commercially available sodium hypochlorite and glutaraldehyde were found to be effective disinfectants for Alginate impression. But hypochlorus add was not adequate disinfectant.