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서지반출
Enhanced UV-Visible Absorbance Detection in Capillary Electrophoresis Using Modified T-Shaped Post-Column Flow Cell
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  • Enhanced UV-Visible Absorbance Detection in Capillary Electrophoresis Using Modified T-Shaped Post-Column Flow Cell
  • Enhanced UV-Visible Absorbance Detection in Capillary Electrophoresis Using Modified T-Shaped Post-Column Flow Cell
저자명
Lim. Kwan-Seop,Kim. Su-Hyeon,Hahn. Jong-Hoon
간행물명
Bulletin of the Korean Chemical Society
권/호정보
2002년|23권 2호|pp.295-300 (6 pages)
발행정보
대한화학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

The construction of the T-shaped post-column flow cell has been changed to enhance the practicability as a UV-visible absorbance detector for capillary electrophoresis. In this new design, a rectangular cube-shaped inner structure is employed, which completely fits the outer rectangular tubing. This arrangement has greatly facilitated the fabrication of the T-cells. In addition, the volume for the auxiliary flow has been dramatically reduced down to 300 ${mu}L$, and its volume flow rate is optimized at 4.2 ${mu}L$/min. The short optical path length in the sheath flows (500 ${mu}m$ on each side) minimizes background absorption, and thus enhances its performance in low-UV wavelengths. We have optimized the auxiliary flow rate at 50 ${mu}m$/s, so that migration times are insensitive to the flow rate. This optimization has improved repeatabilities in migration times and peak heights. A double-beam detection scheme using a pair of photodiodes is employed to increase the signal-to-noise ratio.