Three diene-yn-diene chromophore type compounds were isolated from the flowers of Hemisteptia lyrata Bunge. On the basis of spectral data, isolated compounds were identified as heptadeca-1,7,9,13,15-pentaen-11-yne, 14-acetoxy-2,4,8,10-tetradecatetra-ene-6-yne and 14-hydroxy-2,4,8,10-tetradecatetraene-6-yn. They were first isolated from the genus of Hemisteptia.