This study was designed to research the anti-tumor effect of omija (methanol extract(I), malic acid & ethanol extract(II), and water extract (III)) on human liver cancer cell line SNU-398. MTT assay was used in vitro. The longer thulcorner exposure time and the higher the concentration of Omija extract, the stronger the anti-tumor effect. When the concentration of (II) was 1,600 $mu extrm{g}$/$mell$ and the exposure time reached 96 hours, The strongest propagation inhibition effect occurred with the viability rate as low as 5.06%. $IC_{50}$/ value was 363 $mu extrm{g}$/$mell$. Under the condition of 1,600$mu extrm{g}$/$mell$ and 96 hours, (I) lowered the rate to 7.75%. $IC_{50}$/ value was 489 $mu extrm{g}$/$mell$. When it was 1,600$mu extrm{g}$/$mell$ and 72 hours, (III) the rate decreased to 15.97%. $IC_{50}$/ value was 703 $mu extrm{g}$/$mell$. In all three cases, the viability of the cancer cell decreased significantly when the exposure time ranged between 24 and 48 hours.