The optimal culture conditions in 500$mell$ flask suture, 5$ell$ jar fermenter and 2,000 $ell$ large stale culture were investigated to maximize the production of antibiotic on Rhizoctonia solani AC4, the causal agent of vegetables damping-off, by the strain Bacillus ehimensis YJ-37. Starch (1.5%) as a carbon source, peptone (0.6%) as a nitrogen source and MgC1$_2$(0.15%) as a metal ion in the medium containing N $a_2$HP $O_4$(0.3%) showed the highest production of the antibiotic(s) in a rotary shake (200 rpm). Optimal initial pH of the culture medium, culture temperature and culture time for the antibiotic(s) production were pH 8.0, 32$^{circ}C$ and 54hrs, respertively. Under the optimal renditions in flask culture, cell growth and antifungal activity (clear zone size) were 1.42 $ imes$ 10$^{8}$ cfu/$mell$ (21g-DCW/ $ell$) and 13.9 mm, respectively. In 5 $ell$ jar fermenter (medium 3 $ell$), optimal air flow, agitation speed and culture time for the antibiotic(s) production were 2 vvm, 200 rpm and 48hrs, respectively. Under the optimal conditions in 5 $ell$ jar fermenter, tell growth and antifungal activity were 2.06 $ imes$ 10$^{8}$ cfu/$mell$ (30g-DCW/ $ell$) and 13.4 mm, respectively. Under the culture conditions of air flow (30 vvm) and agitation speed (200 rpm) at 32$^{circ}C$ for 10 days in 2,000 $ell$ large scale culture (medium 1,800 $ell$, pH 8.0), cell growth and antifungal activity were 0.81$ imes$10$^{8}$ cfu/$mell$ (12g-DCW/ $ell$) and 8.6 mm, respectively.