The purpose of this study was to measure distribution of clothing pressure on breast in brassiere according to body shape and motion, and the position of hook-and-eye on brassiere, and to get basic data for comfortable brassiere design. Clothing pressure was measured from 8 female subjects under wearing trials in climatic chamber. When brassiere was tied together with inner or outer hook-and-eye, clothing pressure under the condition were 10.2 and 9.6 gf/cm², respectively. With the degree between main body and arms increased from 0° to 45° and 90°to the front, clothing pressure decreased from 10.2 to 9.6 gf/cm², and then increased to 10.4 gf/cm² When the decree was increased from 45° to 70° to the flank, the pressure increased from 9.3 to 10.6 gf/cm². Fat body shape recorded 10.8 gf/cm², and lean body shape recorded 9.5 gf/cm² of clothing pressure by wearing brassiere. Clothing pressures of brassiere were 7.8g gf/cm² in front, 9.5 gf/cm² in side, 12.8 gf/cm² in the back side. Therefore, clothing pressure of brassiere was influenced to the greater extent by body shape and measuring points on human body than by the position of hook-and-eye and body motion.