The rotational performance off machine tool spindle has a direct influence upon the surface finish of the finished workpiece. This running accuracy of the spindle is improved by increasing preload on the bearings, while it results in higher temperature rise and larger thermal deformation. Therefore, finding the optimal preload condition for high speed spindle is very important factors in spindle motion. in spindle motion. In this study, the effect of the preload on the roundness accuracy has been examined at the different cutting conditions. Experiments were carried out to investigate the effects of the bearing preload on the running accuracy of high speed spindle which was supported by two angular contact bearings.