In order to obtain the information of bio-environment control, the thermal characteristics of soil in the greenhouse heated by the heat pump and latent heat storage system were experimentally analyzed. The experimental systems were composed of the greenhouse with a heat pump and a latent heat storage system (system I), the greenhouse with a heat pump (system II), the greenhouse with a latent heat storage system (system III), and the greenhouse without auxiliary heating system (system IV). The thermal characteristics experimentally analyzed in each system were temperature of soil layers, soil heat storage and release, soil heat capacity and soil heat storage ratio. The results could be summarized as follows. 1. Time to reach the highest temperature at 20cm deep in soil layers of the crop routs in case of system I was shown to be delayed by 6 hours in comparison to the time of the highest temperature at the soil surface. 2. In the clear winter days, the stored heat capacity values fur the system I and the system II were shown to be 22.3% and 11.0% higher than the released heat capacity respectively, and the stored heat capacity values for the system III and the system IV were shown to be 6.2% and 29.6% lower than the released heat capacity respectively This confirms that the system I provided the best heat storage effect. j. The heat quantity values stored or released were shown to be highest at 5 cm depth of soil layers. And it was reduced with increasing of depth of soil layers until 20 cm and was not changed under the soil layer of 20 cm depth. 4. The heat absorption rates of soil, the ratio between supplied and stored heat energy, fur both the system I and system II were lower than 23%.