A modified equation for the evaluation of discomfort of a seated human body exposed to differential vibration at the seat and the floor was proposed in this paper. Through the review and analysis of the preceding studies, effect of phase difference between the seat and the floor vibration on discomfort were quantitatively identified. The phase effect was shown to be governed by not only phase difference between the two vibrations but both their frequency and the magnitude, which means the present equation for the evaluation of perceptual amount of vibration provided by ISO 2631-1 should be modified. The proposed equation was developed such that the correction function was multiplied to the present equation. The correction function consisted of three parts, each of them represented the effect by phase difference, frequency and vibration magnitude on discomfort respectively.