This paper describes an automatic transplantation machine of optical fiber controlled by PLC. The transplantation operations is a key operation for the optical fiber application products, such as bag, cap, and others. To transplant 200∼500 optical fibers, there are many recurrent manual operation needed with conventional process. In this paper. we propose an automatic machine that reduce transplanting time and enhance product quality. Developed machine includes transplantation, heat cutting and ultraviolet coating operation. Also proposed Machine is controlled by PLC to adjust operation parameters such as pulling length, cycle time, coating time and others. Developed Machine has been applied in the field and found to be a useful system.