Content of crude protein in both pileus and stalk increased progresively as growth period became longer and then decreased at old stage. Contents of ash and reducing sugars followed the same trend as that of the crude protein. However, content of crude lipid decreased as growth progressed more. Seventeen kinds of amino acid were detected in Lentinula. Content of total amino acid increased as mushroom grew more, but decreased after old stage. The amino acid such as Glu, His, Gly and Ala were contained with relatively large amount in Lentinula. Content of free amino acid showed the similar trends as that of total amino acid. Contents of most of the mineral increased as growth period progressed, but K content decreased as growth period became longer. Contents of K and P were much higher than contents of other mineral. The major fatty acid contained in Lentinula were linoleicacid, palmitic acid and oleic acid. The large amount of volatile component in Lentinula was 1-octen-3-ol, so that this material was speculated to be major source of perfume. Other perfumic materials such ethyl acetate, 3-octanone, ethanol, (E)-2-octenal, 4-octen-3-one, acetaldehyde, ethyl formate were also contained in Lentinula, especially in pilous.