This study was carried out to find out the effects of date and number of defoliation by different seeding date on growth and yield of perilla. Saeyuepsildlkkae was seeded from April 30 to June 30 at 20 days interval and defoliated from June 30 to Aug. 15 with different defoliation number. 1. Days to emergence, flowering, and maturing were not significantly influenced by defoliation, but those were shortened as seeding date was delayed. The interaction between defoliation and seeding date showed no significant difference. 2. Stem length, number of nodes on main stem, and weight of dry stem were not significantly affected by defoliation, but those were decreased as seeding date was delayed. 3. Yield of fresh loaves was not significantly influenced by seeding date up to May 20, but markedly decreased by the later seeding date. 4. Cluster length and number of cluster per plant were not affected by seeding date, but number of capsules per cluster was decreased from 32 to 25 and 1,000 grain weight was increased from 3.42 to 4.62g as seeding date was delayed. 5. Grain yield was not significantly affected by defoliation and seeding date up to June 10, but markedly decreased by seeding date June 30.