밤잎의 기능성 식품 및 의약품으로서의 활용도를 높이기 위한 일환으로 밤잎의 화학성분, 항산화 및 항균활성을 측정한 결과는 다음과 같다. 일반성분은 총당 11.95%, 조지방 11.50%, 조섬유 10.11%, 조단백질 7.50% 및 회분이 1.79%순으로 나타났으며, 주요 무기성분으로는 Ca과 K으로 그 함량은 각각 215.7 mg%와 196.6 mg%로 나타났다. 비타민 C의 함량은 12.5 mg%였으며, 유리당은 glucose 3.33%, fructose 0.25%, sucrose 0.022% 함유되어 있었다. 밤잎의 주요 지방산은 linoleic acid로 그 함량은 36.86%로 나타났다. 밤나무 잎의 주된 아미노산은 glutamic acid(295.4 mg%), proline(285.7 mgg%), aspartic acid(245.5 mg%), arginine(240.8 mg%), phenylalanine(237.4 mg%) and leucine(230.6 mg%)로 나타났으며, 총 아미노산에 대한 필수아미노산의 비율은 48.3%이었다. 항산화 효과는 용매별 추출물에서는 methanol 추출물이 72.52%, 용매분획 추출물에서는 ethyl acetate 분획물에서 84.12%로 각각 높게 나타났다. 각종 용매별 추출물의 항균활성은 메탄을 추출물이 높게 나타났으며, 용매분획별 추출물에서는 ethyl acetate 분획층에서 다른 분획보다 높게 나타났다.
To study the potential of the chestnut(Castanea crenata S.) leaves, as raw materials for functional food and medicine, chemical components, antioxidative and antimicrobial activities were carried out. The proximate composition was composed of total sugar 11.95%, crude fat 11.50%, crude fiber 10.11%, crude protein 7.50% and ash 1.79% and the components of major minerals were Ca 215.7 mg%, 196.6 mg%. The content of vitamin C wag 12.5 mg% and free sugar was composed of glucose 3.33%, fructose 0.25% and sucrose 0.022%. The major fatty acids in leaves of chestnut were composed of linoleic acid and the amounts of those showed 37.88% area percent. The major amino acids of chestnut leaves were glutamic acid(295.4 mg%), proline(285.7 mg%), aspartic acid(245.5 mg%), arginine(240.8 mg%), phenylalanine(237.4 mg%) and leucine(230.6 mg%). The ratio of essential/total amino acid was 48.3%. Methanol extract and ethyl acetate fraction showed stronger activity of the hydrogen donating activities, each of 72.52 % and 84.12 %, respectively. In solvent extracts using methanol, ethanol, ethyl acetate, chloroform and hexane, methanol extract showed the most effective antimicrobial activities. Antimicrobial activities of ethyl acetate fraction of methanol extract was higher than those of other fractions.
