The purpose of this study is to construct an evaluation criteria based on the relative importance and various combinations of each evaluation index and then to appraise urban biotope in terms of nature experience and recreation. After the standard of evaluation had been developed through the review of existing literature, the relative importance of the evaluation index through expert survey was determined, and the evaluation model to assess biotope value was established. The results of this study are as follows; 1) We have constructed the seventeen evaluation criteria through literature reviews, and have provided relative importance to each evaluation index of the natural in biotope, the diversity of landscape patterns, the appearance-existence of water space, the special landscape elements, and the access based on the results of expert survey. and then we have constructed an evaluation system using the relative importance criteria. 2) To evaluate urban biotope using the evaluation system constructed, we selected 14 sites. The final evaluation results of each site is as follows; The first grade areas are the water space with semi-natural style in front of Gangchon Woobang apartments, the forest zone near Page Temple at Mt. Palgong, the edge area of Jisan and Gosan and the tendril yard against Anshim middle school. The second grade areas are Hyomyung elementary school, the farm land behind the third Anshim Jugong apartment, Ehyun put and the swampy land in front of the Kangchon and Anshim construction site. The third grade areas are Seohan and Hwasung high-rise apartments at Beummul-Dong, the Korea Manpower Agency in the Sungseo industrial zone, and the adjacent area of St. Tongil. The only fourth grade area is the commercial zone near Taegu Department Store. 3) It is highly useful to evaluate urban biotope using the evaluation criteria constructed for this research, the evaluation criteria we developed may be difficult to encompass all expert opinion and the period of space-formation is difficult to estimate urban biotopes. In the future, it is necessary to gather the basic data suitable to estimate biotope and it is necessary to develop detailed evaluation criteria applicable to estimate every biotope that has specific property.