This paper presents a fuzzy rule extraction method using EM(Expectation-Maximization) algorithm and a design method of adaptive neuro-fuzzy control. EM algorithm is used to estimate a maximum likelihood of a GMM(Gaussian Mixture Model) and cluster centers. The estimated clusters is used to automatically construct the fuzzy rules and membership functions for ANFIS(Adaptive Neuro-Fuzzy Inference System). Finally, we applied the proposed method to the water temperature control system and obtained better results with respect to the number of rules and SAE(Sum of Absolute Error) than previous techniques such as conventional fuzzy controller.