본 연구는 불임견의 번식장애를 해결할 목적으로 개 정소상체로부터 채취한 정액을 tris-buffer로 원심분리하여 정장을 제거한 RSP-T 희석 정자의 일반성상과 동결방법 및 glycerol 농도가 동결융해 후 생존성에 미치는 영향과 난자와 정소상체 정자를 매정시켰을 때 정자침입율을 조사하기 위하여 수행하였다. 1. WES, RSP-S 및 RSP-T 희석 정소상체 정액의 정자수는 각각 4.25 $pm$ 0.25(X$10^{6}$ Cells/$mell$), 3.85 $pm$ 0.20(x$10^{6}$cells/$mell$), 4.05 $pm$ 0.28(X$10^{6}$cells/$mell$)이었고, 활력은 50.55 $pm$ 2.75%, 67.25 $pm$2.55%, 78.75$pm$3.55%이었고, 기형정자율은 49.45$pm$2.25%, 37.75 $pm$ 2.10%, 24.25 $pm$ 1.55%이었다. 2. RSP-S와 RSP-T 희석 정소상체 정액을 완만 동결을 했을 때 생존율은 각각 35.00 $pm$2.35%, 45.50 $pm$2.15%와 초급속동결시의 생존율은 16.50 $pm$ 3.55%, 22.55 $pm$ 3.95%이었다. RSP-T 희석 정소상체 정액을 동결시 내동제에 glycerol 농도를 2.0~8.0% 첨가하여 동결했을 때 생존율은 각각 9.25 $pm$ 1.55%~17.50$pm$2.50%로서 신선정액의 25.50% 4.50%~34.00$pm$5.15%에 비해 낮은 생존율을 나타냈다. 3. 동결 융해한 정소상체 희석정자의 평균 수정 능 획득 율과 첨체반응 및 생존정자수는 각각 13.00 $pm$ 2.35%, 3.55 $pm$ 0.85%, 15.50 $pm$ 1.90% 였고, 신선 정소상체 정액의 45.25$pm$5.75%, 7.06 $pm$0.25%, 49.20$pm$6.80%이었다. 신선 및 동결 수정 능 획득 정자를 난자와 매정시켰을 때 난자내 정자의 침입율은 각각 39.25 $pm$4.72 %와 34.24$pm$3.93%였고, 난자당 정자의 침입 수는 각각 1.30$pm$0.33개, 1.10$pm$0.50개이었다.
This study was carried out to investigate the general characteristics such as concentration sperm motility and abnormality of sperm on the whole epididymal semen(EWS), RSP-S(removed seminal plasma by saline) and RSP-T(removed seminal plasma by tris-buffer) semen and survival rates after freezing on motility of whole and RSP-S and RSP-T semen and extender containing 2~8% glycerol, and ability of frozen-thawed sperm to penetrate homologous oocytes. 1. The concentration, motility and abnormality of epididymal WES, RSP-S and RSP-T sperm were 4.25 $pm$ 0.25($ imes$10$^{6}$ Cells/$mell$), 3.85$pm$0.20($ imes$10$^{6}$ Cells/$mell$), 4.05 $pm$ 0.28($ imes$10$^{6}$ Cells/$mell$), 50.55 $pm$ 2.75%, 67.25 $pm$ 2.55%, 78.75 $pm$ 3.55 and 9.45 $pm$ 2.25%, 37.75 $pm$ 2.10%, 24.25 $pm$ 1.55%, respectively. 2. The survival rates of slow and rapid frozen epididymal RSP-S and RSP-T sperm were 35.00 $pm$ 2.35%, 45.50 $pm$ 2.15％ and 16.50 $pm$ 3.55%, 22.55 $pm$ 3.95%, respectively. The survival rate of epididymal WES and RSP-T sperm after freezing following dilution with tris-buffer containing 2~8% glycerol were 9.25 $pm$ 1.55%~17.50 $pm$ 2.50%. 3. The percentage of capacitated and acrosome-reacted sperm prier to culture for fresh and frozen -thawed epididymal RSP-T semen were 45.25 $pm$ 5.75%, 7.06 $pm$ 0.25%, 48.20 $pm$ 6.80% and 13.00 $pm$ 2.35%, 3.55 $pm$ 0.85%, 15.50 $pm$ 1.90%, respectively. The penetration rate the number of sperm per penetrated for fresh and frozen-thawed epididymal RSP-T sperm were 39.25 $pm$ 4.72%, 34.24 $pm$ 3.93% and 1.30 $pm$ 0.33, 1.10 $pm$ 0.50., respectively.
