A soil microorganism producing ${alpha}$- and ${eta}$-glucosidase inhibitors was identified as Bacillus lentimorbus, based on the fatty acid and morphological analyses, along with biochemical and physiological tests. The ${alpha}$-glucosidase inhibitor was highly produced by this strain in a culture medium containing $0.25\%$ of sodium glutamate and $0.5\%$ of glucose, pH 8.0 at $30^{circ}C$ for 2 days. The ${alpha}$-glucosidase inhibitor from culture filtrate of his strain was identified as water soluble, organic solvent nonextractable, and heat stable. In addition to ${alpha}$-glucosidase inhibitor, this strain also produced ${eta}$-glucosidase inhibitor in he same culture medium and this inhibitor showed an antifugal activity against Botrytis cinerea. While the production of ${alpha}$- glucosidase inhibitor was decreased by a glucose concentration higher than $1\%$, the production of ${eta}$-glucosidase inhibitor was lot Influenced by a glucose concentration higher than $20\%$. The ${alpha}$-glucosidase inhibitor from culture filtrate of this strain was separated from the ${eta}$-glucosidase inhibitor through Sephadex G-100 column chromatography.