High temperature sterilization method (30min. at $40^{circ}C$longrightarrow30 min. at $70^{circ}C$longrightarrow30 min. cooling at $20^{circ}C$) was evaluated better than that of chloride, ion water, $O_3$ water, and salt water for the controling of micro-organisms at the early stage of sprout culture. Due to the improved germination rate and effective control of micro-organisms at early stage, the method resulted in much lower in the number of micro-organisms, higher in sprout yield, and 2 days longer in quality keeping at market than sprouts prepared by conventional method. There are two methods related with water temperature; constant temperature method (18.5$pm$ $0.5^{circ}C$) which keeps the same water temperature during the culture and 3-stage temperature method which changes the water temperature depending on the days after culture. Three-stage temperature method set the temperature at 21$pm$ $1^{circ}C$) in the second and third days after the initial acceleration of germination (about 4 hours), at 18.5$pm$0.5$^{circ}C$ in the fourth and fifth days, and at 17.0$pm$ $0.5^{circ}C$) in the sixth and seventh days. Three-stage temperature method could enhance the resistance power to diseases at the early growth stage, control the growth to standard goods (8-9cm in length and 2.15-2.30mm in width), and keep good body color at the middle or final stage. This method also increased the sprout yield by 6% compared with the conventional method, constant temperature method.