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Simultaneous Biocatalytic Synthesis of Panose During Lactate Fermentation in Kimchi
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  • Simultaneous Biocatalytic Synthesis of Panose During Lactate Fermentation in Kimchi
  • Simultaneous Biocatalytic Synthesis of Panose During Lactate Fermentation in Kimchi
저자명
Han. Nam-Soo,Jung. Yoon-Seung,Eom. Hyun-Ju,Koh. Young-Ho,Robyt. John F.,Seo. Jin-Ho
간행물명
Journal of microbiology and biotechnology
권/호정보
2002년|12권 1호|pp.46-52 (7 pages)
발행정보
한국미생물생명공학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

As a functional additive for intestinal microflora, panose ($6^2-{alpha}$-D-glucopyranosylmaltose) was synthesized during kimchi fermentation using the glucose transferring reaction of glucansucrase from Leuconostoc mesenteroides. For the glucose transferring reaction, sucrose and maltose were added ($2\%$ each, w/v) to dongchimi-kimchi as the glucosyl donor and acceptor molecule, respectively. After five days of incubation at $10^{circ}C$, referring to the initial phase for the production of lactic acid in kimchi, over $60\%$ (w/v) of the total sugars were converted into panose and other branched oligosaccharides. Thereafter, the kimchi was stored at $4^{circ}C$ and the amount of panose remained at a constant level for three weeks, thereby indicating the stability of panose to microbial degradation during the period of kimchi consumption. The use of maltose as the acceptor molecule in the kimchi also facilitated a lower viscosity in the kimchi-juice by preventing the synthesis of a dextran-like polymer which caused an unfavorable taste. Accordingly, the application of this new method of simultaneous biocatalytic synthesis of oligosaccharides during lactate fermentation should facilitate the extensive development of new function-added lactate foods.