There are many speech analysis tools available. Among them real-time analysis tool is very useful for interactive experiments. A real-time speech analysis tool was implemented using Matlab. Matlab is a very widely used general purpose signal processing tool. In general, its computational speed is relatively lower than that of the codes from conventional programming languages. Especially, real-time analysis including input of signal and output of the result was not possible in the past. However, due to the improvement of computing power of PCs and inclusion of real-time I/O toolboxes in Matlab, real-time analysis is now possible in some extent by Matlab only. In this experiment, we tried to implement a real-time speech analysis tool using Matlab. Pitch and spectral information is computed in real-time. From the result it is shown that such real-time applications can be implemented easily using Matlab.