Human language activity in the information society has been developing the communication system between humans and machines. The aim of this study was to analyze dialectal speech in Korea. One hundred Kyungsang and one hundred Cholla informants participated in this study. A CSL and Flexible laryngeal fiberscope were used for analysis of the acoustic and glottal gestures of all the vowels and consonants. Test words were made on the picture cards and letter cards which contained each vowel and each consonant, respectively. The dialogue between the examiner and the informants was recorded in a question and answer manner. The acoustic results of two dialects were as follows: Kyungsang and Cholla informants showed neutralization between /e/ and /$varepsilon$. However, the apertures of Kyungsang vowels /i, w, u, o/ were higher than those of Cholla vowels. The /wi/ and /$varepsilon$/ of Kyungsang Diphthong vowels were shown as simple vowels /i/ and /$varepsilon$/ in Cholla dialect. The VOT of Cholla dilaect was longer than that of Kyungsang dialect. The fricative frequence of Kyurlgsang dialect was about 1000Hz higher than that of Cholla dialect. The glottal widths on fiberscopic images showed that the consonant durations of Kyungsang and Cholla dialects were correlated all together with the acoustic duration on the spectrogram.