In this paper, the robust non-fragile guaranteed cost control problem is studied for a class of linear large-scale systems with uncertainties and a given quadratic cost functions. The uncertainty in the system is assumed to be norm-bounded and time-varying. Also, the state-feedback gains for subsystems of the large-scale system are assumed to have norm-bounded controller gain variations. The problem is to design a state feedback control laws such that the closed-loop system is asymptotically stable and the closed-loop cost function value is not more than a specified upper bound for all admissible uncertainties and controller gain variations. Sufficient conditions for the existence of such controllers are derived based on the linear matrix inequality (LMI) approach combined with the Lyapunov method. A parameterized characterization of the robust non-fragile guaranteed cost controllers is given in terms of the feasible solutions to a certain LMI. A numerical example is given to illustrate the proposed method.