This study was carried out to investigate the effect of mixture of Gastrodiae rhizoma (GM) on blood amelioration in high cholesterol-diet rats. Sprague-Dawley male rats were randomly assigned to one normal diet and three high cholesterol-diet groups which contained 1 % (w/w) cholesterol diet. The groups of high cholesterol-diet were classified to control (high cholesterol-diet only), GM-1 (high cholesterol-diet and GM) and ST-1 (high cholesterol-diet and Statin drug). The body and organs weight were not significantly changed among the tested groups. Contents of serum total cholesterol and LDL-cholesterol were significantly increased in cholesterol-diet groups compared with normal diet group but significantly decreased in the group of GM-1. Morphology of red blood cell in GM-1 group was similar to normal diet group but the control group had many crystals of cholesterol. Hepatic xanthine oxidase activity in the rats of high cholesterol-diet was decreased up to the levels of normal diet group according to oral administration of GM. The results of the present study demonstrate that the orally injection of GM can ameliorate the status of total cholesterol and LDL-cholesterol, and repress xanthine oxidase in liver in high cholesterol-diet rats. These finding suggest that GM is expected to be an effective tea for the blood amelioration in high cholesterol-diet rats.