Animation is an art, which experiences through visual and auditory sense. The purpose of this study is to find out whether the theory of film sound can be also applied to animation sound. The study also aims to find out whether the mood and feeling of animation can be influenced by rhythm and fidelity of sound. To begin with, exampled a few animations and analyzed their sound base on the theoretical background of film sound and found out that influence of rhythm and fidelity of sound in animation is much more effective than in film. To find out the relationship between the changes of the mood according to different rhythm, I have applied the various rhythms of sound in a same sequential images. Result proved that the mood of animation was changed according to different rhythms that applied to animation. sound has a great ability of delivering the mood prior to images and different sound can change the overall feeling and mood of animation. Also proved that influence of fidelity of sound is much greater than rhythms of sound, especially in comic animation.