This study was performed to develop accelerated life test method of machanical parts using cumulative damage theory that used to model the fatigue of parts that receive variable load. The cumulative damage theory was introduced, and the estimation of life and calculation of accelerated life test time was illustrated. As the actual application example, accelerated life test method of agricultural tractor transmission was described. Life distribution of agricultural tractor transmission was supposed to follow Weibull distribution and life test time was calculated under the conditions of average life (MTBF) 3,000 hours and 90% reliability for one test sample. According to the cumulative damage theory, because test time can shorten in case increase test load, test time could be reduced by 482 hours when we put the load 1.1 times of rated load than 0.73 times of rated load that is equivalent load calculated by load spectrum of the agricultural tractor. This time, acceleration coefficient was 11.7. This accelerated test method was used to develop accelerated test method of gear reducer, hydraulic hose and bearing as well as agricultural tractor transmission and it is considered to be applied comprehensively to machanical parts the fatigue of which is happened by load or pressure etc.