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Home-built Solid-state NMR Probe for Membrane Protein Studies
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  • Home-built Solid-state NMR Probe for Membrane Protein Studies
  • Home-built Solid-state NMR Probe for Membrane Protein Studies
저자명
Kim. Yong-Ae,Hwang. Jung-Hyun,Park. Jae-Joon
간행물명
Bulletin of the Korean Chemical Society
권/호정보
2003년|24권 9호|pp.1281-1283 (3 pages)
발행정보
대한화학회
파일정보
정기간행물|ENG|
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기타
이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

Proteins in highly oriented lipid bilayer samples are useful to study membrane protein structure determination. Planar lipid bilayers aligned and supported on glass slide were prepared. These stack of glass slide with planar lipid bilayers are not well fit for commercial solid-state NMR probe with round coil. Therefore, homebuilt solid-state NMR probe was built and used for a stack of thin glass plates and RF coil is wrapping directly around the flat square sample. The overall filling factor of the coil is much better and the large surface area enhances the extent to orientation by providing uniform environments for the phospholipids and the high ratio of circumference to area reduces edge effects. $^1H;and;^{15}N$ double resonance probe for 400 MHz NMR (9.4T) with a flat coil (coil size: 11 mm ${ imes}$ 20 mm ${ imes}$ 4 mm) is constructed and tested.