This study was conducted to investigate the subjective degree of fatigue, the degree of fatigue depending on analysis of MMPI and SDNN in middle-aged women. Data were collected from 51 patients, registered at Oriental Medicine Hospital in J-city and K-city respectively. The instrument used in this study were the Multidimensional Assessment of Fatigue developed by Belza(1995) and revised by Jung & Kim(1999) and the Minnesota Multiphasic Personality Inventory(MMPI) and Standard Deviation of Normal to Normal(SDNN). The collected data were analyzed by SPSS/WIN 9.0 program using frequencies, percentage, mean, standard deviation, t-test. The degree of fatigue in middle-aged women: the average score 30.61 out of the total score 40. Subdivision of fatigue scores: common fatigue degree 7.71, distress due to fatigue 7.67, degree of daily activity fatigue 7.43, and fatigue frequency in the previous week 7.84. The degree of fatigue according to subjective recognition was shown significant difference(t=6.707, p=.000).: group of recognition (33.58), group of ignorance (23.47). The degree of fatigue according to MMPI was shown significant difference. : In the group of recognition, scales of Hs, D, Hy, Pt were higher. The degree of fatigue according to SDNN was shown significant difference (T=-4.062, P=.000). : group of recognition (33.47ms), group of ignorance (45.70ms). The total fatigue score and scales of Hs, D, Hy in MMPI was correlated positively (r=.576; r=.466; r=.518), and total fatigue score and SDNN was correlated negatively (r=-.576). Conclusively fatigue is one of difficult problems in middle-aged women. Therefore it is necessary to develope some nursing intervention for reducing fatigue in its early stages.