The antioxidative constituents isolated from Hedyotis diffusa were identified as quercetin 3-O-${eta}$-rutinoside (1) and quercetin 3-O-${eta}$-glucoside (2). We also isolated asperuloside (3) from this plant. Identification was done based on spectroscopic analysis. Quercetin 3-O-${eta}$-rutinoside was the stronger antioxidant than quercetin 3-O-${eta}$-glycoside while asperuloside was inactive.