This study is to establish the quality control method of Adenophorae Radix. (6R,7R)-E,E-tetradeca-4,12-diene-8,10-diyne-1,6,7-triol (1) isolated from this plant was adequate as an analytical marker. Content of 1 in Adenophorae Radix was $0.006;{pm};0.003%$ (n = 43). In addition, total ash content was $6.5;{pm};4.0%$, and loss on drying was $12.1;{pm};2.1%$.