Cynanchi Wilfordii Radix has been used for the treatment of prematurely grey hair, bald and constipation and Polygoni Multiflori Radix has been used for the treatment of weakness, knee pain, premature greying, elevated serum cholesterol, coronary heart disease, naurasthenia and insominia. Pattern recognition for the analysis of Cynanchi Wilfordii Radix and Polygoni Multiflori Radix was conducted using HPLC method. Pattern of Cynanchi Wilfordii Radix and Polygoni Multiflori Radix was different and we distinguished two medicinal plants by HPLC.