This study was carried out to evaluate cytotoxic effects of Houttuynia cordata $T_{HUNB}$ extracts on A549(lung cancer), B16(mouse melanoma), MDA-MB231(breast cancer) and SNU-C4(colon cancer) cell lines. We have determined by 3-(4, 5-dimethylthiazol-2-Yl)-2, 5-diphenyl-2H-tetrazoliumbromide(MTT) assay. The $150{mu}g/ml$ concentration of chloroform extract of Houttuynia cordata $T_{HUNB}$ was shown significantly antitoxic activity on MDA-MB231$(65.96{pm}5.68%)$ and SNU-C4$(55.94{pm}7.39%)$ cell lines.